National Research Database of Zimbabwe

Short Communication:The use of polyethylene glycol in the diagnosis of polyphenolics in the foliage of Acacia species.

Mokoboki, H.K (2011) Short Communication:The use of polyethylene glycol in the diagnosis of polyphenolics in the foliage of Acacia species.

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Abstract

The aim of this work was to assess the efficacy of polyethylene glycol (PEG) for measuring tannin content in leaves of species, Acacia karroo, Acacia nilotica, Acacia tortilis, Acacia galpinii, Acacia sieberiana, Acacia hebeclada, and Acacia rhemniana. In addition, in vitro dry matter and organic matter digestibility of the browse leaves were assessed. Young leaves from the species were collected from five different trees of each species at the University of Limpopo in April 2002, and their capacity to bind PEG was compared using a completely randomized design. PEG (MW 4000) was added at ratios of plant material: PEG solution (w/v) of 1:05, 1:10, 1:15, 1:20 and 1:25. The ratio of 1: 20 (w/v) resulted in the best response in terms of tannin precipitated compared to the other ratios. The PEG binding capacity of Acacia species was found to be high ranging from 12.73 to 28.82 % dry matter, in direct response to the proportion of tannin content in the leaves. Dry matter and organic matter digestibility varied from 345.4 to 534.2 and 254.4 to 473.9 g/kg DM, respectively, for all species and were negatively correlated to PEG-binding capacity. A. hebeclada, A. sieberiana and A.galpinii had trace levels of total phenolics, A. tortilis contained approximately 90 g/kg DM of total phenolics whilst A. karroo, A. nilotica and A. rhemniana had intermediate amounts. These results suggested that polyethylene glycol can be used to determine the content and effects of tannins in tanniferous plants used by ruminants.

Item Type: Article
Uncontrolled Keywords: Polyethylene glycol binding capacity.,Tropical forages,Tannin assessment,in vitro degradability
Subjects: UNSPECIFIED
Divisions: Universities > State Universities > National University of Science and Technology
Depositing User: Mr. Evans Katiyo
Date Deposited: 01 Dec 2015 00:31
Last Modified: 01 Dec 2015 00:31
URI: http://researchdatabase.ac.zw/id/eprint/1207

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