Phenotyping of the glutathione S-transferase M1 polymorphism in Zimbabweans and the effects of chloroquine on blood glutathione S-transferases M1-A

Mukanganyama, Stanley (1997) Phenotyping of the glutathione S-transferase M1 polymorphism in Zimbabweans and the effects of chloroquine on blood glutathione S-transferases M1-A.

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Official URL: http://ir.nust.ac.zw/xmlui/handle/123456789/501

Abstract

Clinica Chimica Acta 265 (1997) 145-155,The frequency of the null allele phenotype of glutathione S-transferase (GST) M1 was investigated in 114 Zimbabweans and results for a subset of 63 subjects were compared with genotyping by PCR. In addition, the effect of the antimalarial chloroquine on blood levels of GSTMl and GSTA in 19 subjects was studied. Quantification of GSTs was by enzyme linked immunosorbent assays (ELISA). Thirty percent of the subjects were of the GSTMl null phenotype. Comparison of results of phenotyping by ELISA and genotyping by PCR showed that 16% of samples were in discordance; unknown mutations in the GSTMl gene in the Zimbabwean population may explain this observation. Chloroquine decreased levels of blood GSTM1 and GSTA by 50% or more. In populations treated with chloroquine, these decreases in GST activities might lead to compromised ability to detoxify xenobiotics, could confound GSTMl phenotyping and might invalidate use of GSTA as an indicator of liver damage. O 1997 Elsevier Science B.V. Keywords: Glutathione S-transferases; Phenotype; Black Zimbabweans; Chloroquine,The International Program in Chemical Sciences (lpics) Uppsala University. Sweden and the University of Zimbabwe Research Board.

Item Type: Article
Uncontrolled Keywords: Glutathione S-transferases,Phenotype;,Black Zimbabweans,Chloroquine
Divisions: Universities > State Universities > National University of Science and Technology
Depositing User: Mr. Edmore Sibanda
Date Deposited: 01 Dec 2015 01:02
Last Modified: 01 Dec 2015 01:02
URI: http://researchdatabase.ac.zw/id/eprint/1249

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