Kashangura, Chenjerayi (2007) LOW – COST MEDIA FORMULATIONS FOR CULTURE MAINTENANCE AND PROLIFIC FRUITING OF OYSTER MUSHROOMS (PLEUROTUS SPP.). pp. 1-13. ISSN 1818-3794
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Abstract
Modified media for Pleurotus sajor-caju and Pleurotus ostreatus was formulated and investigated in terms of its suitability and effect on the mycelial growth rate of the Pleurotus spp. strains. The modified media was of various whole-wheat grain: media (Malt extract agar[MEA] or Potato dextrose agar [PDA]) ratios of 1:1 to 1:5. Polyethylene (PEG) 6000 was also added as its effects on growth rate at minute concentrations were noted in earlier work. The 1:1 ratio mixture resulted in the drying of the wheat grain by the 8th day. The water content of the media formulations were all significantly different from the water content of conventional Malt Extract Agar (MEA, 3 %) (97.9 % water) and Potato dextrose Agar (PDA, 3 %) (97.9 % water). The water contents generally ranged from 14.3 % (1:2 MEA) to 57.4 % (1:4 PDA). There was no clear-cut trend for the water contents of the media formulations. The growth rate generally initially decreased below that obtained for normal media (MEA and PDA). However the growth rate increased above that for normal media for the 1:2 to 1:5 for MEA and 1:3 to 1:5 for PDA. The water content of the mixtures cannot be used to predict the growth rates. Generally mushrooms are taken to require adequate water however surprisingly growth higher than that for normal media was obtained for media with low water content. The increase in growth rate is attributed to the richness in terms of nutrients. PEG amended MEA media generally resulted in higher growth rates than non-amended media. This has been the case in earlier work done. The crushed wheat grain : media formulations ranging from 1:2 to 1:15 with PDA and no PDA treatments (the media formulation comprised only of distilled water and crushed grain) induced luxuriant mycelial growth and prolific fruiting of the Pleurotus spp. The water content of the crushed wheat grain: media formulations ranged from 75 % to 97 %. Water content generally increased from 1: 2 media to 1:15 media. Growth rate was highest for 1: 5 + PDA media. 1:2 – PDA had the lowest growth rate. The low –cost media formulations 1:3 to 1:5 (whole wheat grain) and 1:3 to 1:15 (crushed wheat grain) have potential use as low-cost alternatives to culture Pleurotus spp. for culture maintenance, spawn production, mushroom cultivation and bioremediation.
| Item Type: | Article |
|---|---|
| Subjects: | Q Science > Q Science (General) Q Science > QR Microbiology |
| Depositing User: | Dr Chenjerayi Kashangura |
| Date Deposited: | 25 Sep 2017 07:52 |
| Last Modified: | 25 Sep 2017 07:52 |
| URI: | http://researchdatabase.ac.zw/id/eprint/3497 |
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